000 05173nam a22005175i 4500
001 978-981-13-0785-0
003 DE-He213
005 20200712165718.0
007 cr nn 008mamaa
008 181111s2018 si | s |||| 0|eng d
020 _a9789811307850
_9978-981-13-0785-0
024 7 _a10.1007/978-981-13-0785-0
_2doi
050 4 _aQD431-431.7
072 7 _aPSBZ
_2bicssc
072 7 _aSCI007000
_2bisacsh
072 7 _aPSB
_2thema
082 0 4 _a572.6
_223
100 1 _aPunekar, N.S.
_eauthor.
_4aut
_4http://id.loc.gov/vocabulary/relators/aut
245 1 0 _aENZYMES: Catalysis, Kinetics and Mechanisms
_h[electronic resource] /
_cby N.S. Punekar.
250 _a1st ed. 2018.
264 1 _aSingapore :
_bSpringer Singapore :
_bImprint: Springer,
_c2018.
300 _aXXIII, 562 p. 263 illus., 76 illus. in color.
_bonline resource.
336 _atext
_btxt
_2rdacontent
337 _acomputer
_bc
_2rdamedia
338 _aonline resource
_bcr
_2rdacarrier
347 _atext file
_bPDF
_2rda
505 0 _aPart I: Enzyme catalysis - a perspective -- Enzymes -Their place in Biology -- Enzymes - Historical aspects -- Exploiting enzymes – Technology -- On enzyme nomenclature and classification -- Hallmarks of an enzyme catalyst -- Origins of enzyme catalytic power -- Origins of enzyme catalytic power -- Which enzyme uses what trick? Some remarks -- Structure and catalysis: Conformational flexibility and protein motion -- Part II: Enzyme kinetic practice and measurements -- Chemical kinetics – Fundamentals -- Concepts of equilibrium and steady-state -- ES complex and pre-steady-state kinetics -- Principles of enzyme assays -- Good kinetic practices -- Quantification of catalysis and measures of enzyme purity -- Henri-Michaelis-Menten equation -- More complex rate expressions -- Enzyme kinetic data - Collection and analysis -- Part III: Elucidation of Kinetic mechanisms -- Approaches to kinetic mechanism – Overview -- Analysis of initial velocity patterns -- Enzyme inhibition analyses -- Irreversible inhibitions -- Reversible inhibitions -- Alternate substrate (product) interactions -- pH Studies with enzymes -- Isotopes in enzymology -- Isotope exchanges at equilibrium -- Isotope effects in enzymology -- From kinetic data to mechanism and back -- Part IV: Chemical mechanisms and catalysis -- Chemical reactivity and molecular interactions -- Acid-base chemistry and catalysis -- Nucleophilic catalysis and covalent reaction intermediates -- Phosphoryl group chemistry and importance of ATP -- Enzymatic oxidation-reduction reactions -- Carboxylations and decarboxylations -- Electrophilic catalysis and amino acid transformations -- Integrating kinetic and chemical mechanisms – A synthesis -- Part V: Frontiers in enzymology -- Regulation of enzyme activity -- In vitro versus in vivo - Concept and consequences -- Future of enzymology – An appraisal -- Closure - Whither Enzymology.
520 _aThis enzymology textbook for graduate and advanced undergraduate students covers the syllabi of most universities where this subject is regularly taught. It focuses on the synchrony between the two broad mechanistic facets of enzymology: the chemical and the kinetic, and also highlights the synergy between enzyme structure and mechanism. Designed for self-study, it explains how to design enzyme experiments and subsequently analyze the data collected. The book is divided into five major sections: 1] Introduction to enzymes, 2] Practical aspects, 3] Kinetic Mechanisms, 4] Chemical Mechanisms, and 5] Enzymology Frontiers. Individual concepts are treated as stand-alone chapters; readers can explore any single concept with minimal cross-referencing to the rest of the book. Further, complex approaches requiring specialized techniques and involved experimentation (beyond the reach of an average laboratory) are covered in theory with suitable references to guide readers. The book provides students, researchers and academics in the broad area of biology with a sound theoretical and practical knowledge of enzymes. It also caters to those who do not have a practicing enzymologist to teach them the subject.
650 0 _aEnzymology.
650 0 _aProteins .
650 0 _aBiomedical engineering.
650 0 _aMicrobiology.
650 1 4 _aEnzymology.
_0http://scigraph.springernature.com/things/product-market-codes/L14070
650 2 4 _aProtein-Ligand Interactions.
_0http://scigraph.springernature.com/things/product-market-codes/L14060
650 2 4 _aBiomedical Engineering/Biotechnology.
_0http://scigraph.springernature.com/things/product-market-codes/B24000
650 2 4 _aApplied Microbiology.
_0http://scigraph.springernature.com/things/product-market-codes/C12010
650 2 4 _aProtein Structure.
_0http://scigraph.springernature.com/things/product-market-codes/L14050
710 2 _aSpringerLink (Online service)
773 0 _tSpringer eBooks
776 0 8 _iPrinted edition:
_z9789811307843
776 0 8 _iPrinted edition:
_z9789811307867
856 4 0 _uhttps://doi.org/10.1007/978-981-13-0785-0
912 _aZDB-2-SBL
999 _c17680
_d17680
942 _cebook